Summary
Type: SPE
Application #: 304540
Catalog#: 730464 - SPE Cartridges, Chromabond, PSA, 6ml, PP, 1000mg, 30/pk
Detection: 50 µL of the elution were injected into the HPLC system without further concentration. HPLC analysis was performed using an C18 column (e.g. EC 150/4.6NUCLEODUR® 100-5 C18ec; Cat.-No. 760008.46) by is
Conditions: Column conditioning:
3mL of methanol and 5mL of a 0.05 mol/L Na2HPO4 solution of pH 9.0
Sample aspiration:
Twenty-five millilitres of spiked beer were passed through the cartridge
Column washing:
the cartridge was washed with 5mL of water and with 5mL of a 0.05 mol/L Na2HPO4 solution of pH 9.0
Elution:
Finally, the analytes were eluted with 25mL of a solution containing 0.05 mol/L Na2HPO4 solution of pH 9.0, methanol and acetonitrile (60:20:20).
Authors: SPE department
Source: Macherey-Nagel